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Cloning, expression and bioactivity of wild ruddy shelduck (Tadorna ferruginea) interleukin-2

Posted on 15. September, 2014.

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Interleukin-2 (IL-2) is one of the most actively studied interleukins; the data on its involvement in the regulation of immune functions confirm its role as a T-cell growth factor. The IL-2 is a powerful growth factor for variety of cell types, plays a central role in the cell differentiation, and stimulates the activity of many kinds of killer cells.

IL-2 not only affects the growth and differentiation of helper and cytotoxic T cells, but it also that of B cells, natural killer cells, lymphokine-activated killer cells, oligodendrocytes, monocytes, and macrophages, and it is involved in the secretion of immunoglobulin. Moreover, IL-2 can stimulate the expression of the MHC II-type antigen, transferrin receptor, insulin receptor, and production of many cytokines, such as interferon-γ (IFN-γ), IL-4, IL-5, IL-6, TNF-α, and TNF-β. IL-2 has been used as an important adjuvant for inactivated or subunit viral vaccines, for example, in production of herpes simplex, rabies and hepatitis B vaccines. It may also have a role in the protection against bacterial and viral diseases. 

Since the human IL-2 gene was first cloned and sequenced, IL-2 genes for more than 30 animal species have been cloned and identified. IL-2 genes of poultry including chicken, turkey, goose and duck have been extensively cloned and expressed. The previous studies on molecular signatures and bioactivity of birds’ interleukin-2 were mainly focused on domestic fowl and some wild birds from non-migratory or captive population. However, little information is available on the IL-2 genes in migratory waterfowl which are ecologically important species potentially involved in virus transmission. 

The objectives of this study were to clone the cDNA sequence of wild ruddy shelduck IL-2, to express RSIL-2 mature protein in vitro, and to detect the bioactivity of the recombinant ruddy shelduck IL-2 (rRSIL-2) protein in vitro. This study brought some new information on the IL-2 of anseriformes birds.

Read the full article in Avian Biology Research, Volume 7, Number 3, August 2014, pp. 146-152.

Authors: Lanlan Liu, Xianyun Xu, Ruijun Hao, Chunyan Han, Yanna Zhuang, Chao Yan and Xiangwei Zeng

Keywords: ruddy shelduck, interleukin-2, cloning, expression, lymphocyte proliferation


Image courtesy of Sokolov Alexey /